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PI4KB promotes BC cells proliferation and invasion. (A) The expression of PI4KB was knocked down by <t>siRNA.</t> (B, C) CCK‐8 assay and colony formation showed that the proliferative ability of BC cells decreased after knockdown of PI4KB . (D, E) Transwell and wound healing experiments showed that the migration ability of BC cells decreased after knocking down PI4KB . The above experiments were repeated three times, and the expression of PI4KB was analyzed by one‐way ANOVA. Other experiments were analyzed by t ‐test. p < 0.05 was statistically significant. (* p < 0.05; ** p < 0.01). BC, breast cancer.
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PI4KB promotes BC cells proliferation and invasion. (A) The expression of PI4KB was knocked down by <t>siRNA.</t> (B, C) CCK‐8 assay and colony formation showed that the proliferative ability of BC cells decreased after knockdown of PI4KB . (D, E) Transwell and wound healing experiments showed that the migration ability of BC cells decreased after knocking down PI4KB . The above experiments were repeated three times, and the expression of PI4KB was analyzed by one‐way ANOVA. Other experiments were analyzed by t ‐test. p < 0.05 was statistically significant. (* p < 0.05; ** p < 0.01). BC, breast cancer.
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PI4KB promotes BC cells proliferation and invasion. (A) The expression of PI4KB was knocked down by siRNA. (B, C) CCK‐8 assay and colony formation showed that the proliferative ability of BC cells decreased after knockdown of PI4KB . (D, E) Transwell and wound healing experiments showed that the migration ability of BC cells decreased after knocking down PI4KB . The above experiments were repeated three times, and the expression of PI4KB was analyzed by one‐way ANOVA. Other experiments were analyzed by t ‐test. p < 0.05 was statistically significant. (* p < 0.05; ** p < 0.01). BC, breast cancer.

Journal: Journal of Cell Communication and Signaling

Article Title: Paraptosis‐related genes regulate tumor immune microenvironment and predict prognosis in breast cancer

doi: 10.1002/ccs3.70056

Figure Lengend Snippet: PI4KB promotes BC cells proliferation and invasion. (A) The expression of PI4KB was knocked down by siRNA. (B, C) CCK‐8 assay and colony formation showed that the proliferative ability of BC cells decreased after knockdown of PI4KB . (D, E) Transwell and wound healing experiments showed that the migration ability of BC cells decreased after knocking down PI4KB . The above experiments were repeated three times, and the expression of PI4KB was analyzed by one‐way ANOVA. Other experiments were analyzed by t ‐test. p < 0.05 was statistically significant. (* p < 0.05; ** p < 0.01). BC, breast cancer.

Article Snippet: Transient small interfering RNA (siRNA) transfection was used to knock down PI4KB , with transfection reagents purchased from Yeasen (Shanghai, China).

Techniques: Expressing, CCK-8 Assay, Knockdown, Migration